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Question

红豆杉细胞系合成紫杉醇的能力低且不能稳定传代是限制其不能大规模培养的主要原因。迄今为止，红豆杉细胞培养生产紫杉醇已达到中试水平，但要实现大规模生产，关键是要提高培养物产量和紫杉醇含量，选择高产细胞系。因此建立稳定的高产细胞株及其稳定保存技术是工业化生产的前提。
本研究的目的是通过加入前体筛选,紫外诱变筛选出紫杉醇高产细胞株，取得的主要结果如下：
(1) 红豆杉细胞对于前体苯丙氨酸的耐受程度较高，影响较缓慢而且持续时间长，当苯丙氨酸浓度达到4.0mmol/L时，继代培养不能再生长；因而最适筛选浓度为3.0mmol/L。
(2) 红豆杉细胞对于对于前体苯甲酸钠的耐受程度较低，影响迅速而且持续时间短，当苯甲酸钠浓度为0.5mmol/L时，细胞生长的较好，但其促进作用不明显，苯甲酸钠浓度为1.5mmol/L时，细胞无法生长。苯甲酸钠浓度为1.0mmol/L时，继代培养不能再生长，苯甲酸钠浓度为0.5mmol/L，继代培养能再生长，时因而最适筛选浓度为0.5mmol/L左右。
(3) 紫外诱变结合前体筛选红豆杉高产细胞系有一定可行性，苯甲酸钠为0 mmol/L时有新红豆杉细胞长出；但具有一定的随机性，故不能肯定。

注 ：紫杉醇 taxol 红豆杉 taxus 苯丙氨酸 phenylalanine 苯甲酸钠 benzoate 前体 precursor 有些不好搞的就意译
谢谢啦 翻译好的再给分100

Answer 1

精心手工翻译，希望对您有所帮助。

红豆杉细胞系合成紫杉醇的能力低且不能稳定传代是限制其不能大规模培养的主要原因。迄今为止，红豆杉细胞培养生产紫杉醇已达到中试水平，但要实现大规模生产，关键是要提高培养物产量和紫杉醇含量，选择高产细胞系。因此建立稳定的高产细胞株及其稳定保存技术是工业化生产的前提。
The poor ability of taxus clone to integrate taxol and incapable stable passage is the major reason to limit its large-scale cultivation. So far, taxol which is cultivated and produced by taxus clone has reached the level of pilot scale, but the key is improving the output of culture and taxol by choosing high yielding clone in order to realize the large-scale manufacturing. Therefore, the industrial manufacturing is based on the premise of the building of stable high yielding cell strains and the stable storage technique.

本研究的目的是通过加入前体筛选,紫外诱变筛选出紫杉醇高产细胞株，取得的主要结果如下：
The goal of this study is to filter the high yielding taxol cell strains from ultraviolet mutagenesis by adding precursor screening. The acquired major results are as follows:

(1) 红豆杉细胞对于前体苯丙氨酸的耐受程度较高，影响较缓慢而且持续时间长，当苯丙氨酸浓度达到4.0mmol/L时，继代培养不能再生长；因而最适筛选浓度为3.0mmol/L。
(1) The taxus cells have relative high levels of endurement for precursor phenylalanine, and are impacted rather slowly and have long lasting period. The successive transfer culture is unable to grow when the consistency of phenylalanine reaches 4.0mmo/L. So the most suitable consistency for filtering is 3.0mmol/L.

(2) 红豆杉细胞对于对于前体苯甲酸钠的耐受程度较低，影响迅速而且持续时间短，当苯甲酸钠浓度为0.5mmol/L时，细胞生长的较好，但其促进作用不明显，苯甲酸钠浓度为1.5mmol/L时，细胞无法生长。苯甲酸钠浓度为1.0mmol/L时，继代培养不能再生长，苯甲酸钠浓度为0.5mmol/L，继代培养能再生长，时因而最适筛选浓度为0.5mmol/L左右。
(2) The taxus cells have relative low levels of endurement for precursor benzoate and are affected rather fast with short lasting period. The cells grow well when the consistency of benzoate is 0.5mmol/L, but it is not obvious for the promotion effect. The cells stop growing when the consistency of benzoate reaches 1.5mmol/L. And the successive transfer culture is unable to grow when the consistency of benzoate is at 1.5mmol/L whereas it grows at 0.5mmol/L. Therefore, the perfect consistency for filtering is around 0.5mmol/L.

(3) 紫外诱变结合前体筛选红豆杉高产细胞系有一定可行性，苯甲酸钠为0 mmol/L时有新红豆杉细胞长出；但具有一定的随机性，故不能肯定。
It is possible to get high yielding taxus cell strains by combining ultraviolet mutagenesis with precursor screening since when the consistency of benzoate is at 0mmol/L there is new taxus cells growing; but it is not confirmed yet because of the randomness.

Answer 2

Taxus paclitaxol's ability to low and not stablizable passaging is to limit its cannot be the main causes of mass culture.to date, Taxus taxol production has been reached in the trial level, but you want to implement large scale production, the key is to improve the training of yield and taxinol content, select high - yielding cell lines.therefore establishing stable high - yield cell lines and stable storage technique is the premise of industrial production.
This study aims by joining precurson uliraviolet mutagenizing, screening out taxinol high - yielding cell lines, main findings are as follows:
(1) of Taxus for phenylalamine chilosoyllium precurson, more slowly and duration, when concentrations 4.0mmol/l pheyalanine, stepra training cannot be re - growth; thus the optimum concentration for 3.0mmol/l.
(2) of Taxus for for precurson sodium benzoate tolerance less immediate and short duration, when sodium benzoate concentration for 0.5mmol/l, hepatocytegrowth better, but it's not obvious, thecloudcluster hydroxybenzoate natrium 1.5mmol/l, the concentration is unable to cell growth.sodium benzoate concentration for 1.0mmol/l, stepra training cannot be re - growth, sodium benzoate concentration for 0.5mmol/l, stepra training can be re - growth, resulting in optimum concentration for 0.5mmol/l.
(3) UV－second－order mutigenicity hongdoushan precurson screening combined with high - yielding cell lines have the possibility, sodium benzoate is 0 mmol/l hongdoushan tbinit new cells to grow; but with a certain amount of randomicity, it cannot be sure.

Note: hongdoushan taxinol taxol Taxus pheyalanine precurson hydroxybenzoate sodium benzoate phenylalanine precursor some rightok longcalledforsecretary in respect of translation and translation good thanks again to 100

Answer 3

Synthesis of cell lines of Taxus paclitaxel's ability to stabilize low and can not limit its passage is not the main reason for the large-scale cultivation. To date, the paclitaxel production of Taxus cell cultures have reached a level in the test, but in order to achieve large-scale production, the key is to enhance the culture of taxol production and content, select high-yielding cell lines. Therefore the establishment of a stable high-yield and stable cell line to preserve the production technology is a prerequisite for industrialization.
The purpose of this study is the precursor by adding filter, UV-induced taxol selected high-yielding cell lines, the main results obtained are as follows:
(1) Taxus cell precursors for phenylalanine tolerated a higher degree of impact than the slow and lasted for a long time, when the phenylalanine concentration of 4.0mmol / L, the subculture can not be re-growth; thus the most appropriate screening concentration of 3.0mmol / L.
(2) Taxus cell precursors for the tolerance of sodium benzoate less impact on the rapid and short duration, when the concentration of sodium benzoate 0.5mmol / L, the cell growth better, but its role was not obvious, sodium benzoate concentration of 1.5mmol / L, the cells can not grow. Sodium benzoate concentration of 1.0mmol / L, the following can not be re-growth culture, sodium benzoate concentration of 0.5mmol / L, another subculture growth, and thus the optimal filter when the concentration of 0.5mmol / L or so.
(3) The combination of UV-induced pre-screening high-yielding cell lines of Taxus have the possibility of sodium benzoate for 0 mmol / L when the new cells grow yew; but with a certain degree of randomness, it can not be sure.

Answer 4

Synthesis of cell lines of Taxus paclitaxel's ability to stabilize low and can not limit its passage is not the main reason for the large-scale cultivation. To date, the paclitaxel production of Taxus cell cultures have reached a level in the test, but in order to achieve large-scale production, the key is to enhance the culture of taxol production and content, select high-yielding cell lines. Therefore the establishment of a stable high-yield and stable cell line to preserve the production technology is a prerequisite for industrialization. The purpose of this study is the precursor by adding filter, UV-induced taxol selected high-yielding cell lines, the main results obtained are as follows: (1) Taxus cell precursors for phenylalanine tolerated a higher degree of impact than slow and lasted for a long time, when the phenylalanine concentration of 4.0mmol / L, the subculture can not be re-growth; therefore the optimal concentration for selection 3.0mmol / L. (2) (2) Taxus cell precursors for the tolerance of sodium benzoate less impact on the rapid and short duration, when the concentration of sodium benzoate 0.5mmol / L, the cell growth better, but its role was not obvious, sodium benzoate concentration of 1.5mmol / L, the cells can not grow. Sodium benzoate concentration of 1.0mmol / L, the following can not be re-growth culture, sodium benzoate concentration of 0.5mmol / L, another subculture growth, and thus the optimal filter when the concentration of 0.5mmol / L or so. (3) The combination of UV-induced pre-screening high-yielding cell lines of Taxus have the possibility of sodium benzoate for 0 mmol / L when the new cells grow yew; but with a certain degree of randomness, it can not be sure.