李光鹏的主要学术成就

如题所述

1.世界上首例克隆骡子--2003 年3匹克隆骡问世,成果发表于 ?科学? 杂志。
2.2000年获得世界上首例初级卵母细胞核移植后的哺乳动物个体--兔。
3.2001 年获得世界上首例次级卵母细胞纺锤体交换后的哺乳动物个体--小鼠。
4.1998年获得世界上第二例胚胎细胞克隆猪。
5.数百头克隆牛的创造者。
6.异种克隆大熊猫的主要参入者. 1999年度国家十大科技新闻之一。
7.共发表论文70多篇, 其中SCI 论文28篇;主编及参编学术著作4部; 参与翻译专著一部。
研究方向:
动物克隆;核质关系;减数分裂调控; 干细胞研究;转基因动物。
发表论著与论文:
自1990年至今,共在国内外学术期刊上发表论文70多篇, 其中SCI 论文28篇;主编及参编学术著作4部; 参与翻译专著一部。
著作:
1.主编:“发育生物学原理与胚胎工程” 黑龙江科学技术出版社 1995;
2. 副主编:“受精生物学----受精机制与生殖工程”。(陈大元主编;孙青原,李光鹏 副主编)。科学出版社 2001;
3. 参编:“哺乳动物胚胎学” (秦鹏春主编)。科学出版社 2001;
4. White KL, Woods GL, Vanderwall DK, Li GP, Bunch TD. Cloning the Equine. In: Epigenetic Risks of Cloning. Edited by Akio Inui, 2006. pp 59-70. CRC Press, Taylor & Francis Group, Boca Raton, Florida, USA;
5. 参译: “小鼠胚胎操纵实验指南”. (孙青原, 陈大元 主译). 化学工业出版社. 2006。
近几年主要研究论文:
1.Ying Liu, Guang-Peng Li, Benjamin R. Sessions, Lee F. Rickords, Kenneth L. White and Thomas D. Bunch. Nicotine Induces Multinuclear Formation and Causes Aberrant Embryonic Development in Bovine. Mol Reprod Dev. 2007, in press.
2.Liu Y, Li GP, White KL, Rickords LF, Sessions BR, Aston KI, Bunch TD. Nicotine alters bovine oocyte meiosis and affects subsequent embryonic development. Mol Reprod Dev. 2007 Apr 17; [Epub ahead of print], PMID: 17440977 [PubMed - as supplied by publisher].
3.Pate BJ, White KL, Winger QA, Rickords LF, Aston KI, Sessons BR, Li GP, Campbell KD, Weimer B, Bunch TD. 2007. Specific integrin subunits in bovine oocytes, including novel sequences for alpha 6 and beta 3 subunits. Mol Reprod Dev. 2007 May;74(5):600-7.
4.Liu Y, Li GP, Rickords LF, White KL, Sessions BR, Aston KI, Bunch TD. Effect of nicotine on in vitro maturation of bovine oocytes. Anim Reprod Sci. 2006 Nov 26; [Epub ahead of print] PMID: 17178443 [PubMed - as supplied by publisher]
5.Kenneth I. Aston, Guang-Peng Li, Brady A. Hicks, Benjamin R. Sessions, Barry J. Pate, Douglas S. Hammon, Thomas D. Bunch and Kenneth L. White. 2006. The developmental competence of bovine nuclear transfer embryos derived from cow versus heifer cytoplasts. Animal Reproduction Science. Animal Reprod Science. 95(3-4):234-243.
6.Guang-Peng Li, Thomas D. Bunch, Kenneth L. White, Lee Rickords, Ying Liu and Benjamin R. Sessions. 2006. The denuding and centrifugation of maturing bovine oocytes alters oocyte spindle integrity and the ability of cytoplasm to support parthenogenetic and nuclear transfer embryo development. Mole Reprod Dev. 73: 446-451.
7.Aston KI, Li GP, Hicks BA, Sessions BR, Pate BJ, Hammon D, Bunch TD and White KL. 2006. Effect of the time interval between fusion and activation on nuclear state and development in vitro and in vivo of bovine somatic nuclear transfer embryos. Reproduction 131:45-51.
8.Guang-Peng Li, Ying Liu, Thomas D. Bunch, Kenneth L. White, Kenneth I. Aston. 2005. Asymmetric division of spindle microtubules and microfilaments during bovine meiosis from metaphase I to metaphase III. Mol. Reprod. Dev. 71: 220-226.
9.G-P Li, Y Liu, KL White, TD Bunch. 2005. Cytogenetic analysis of diploidy in cloned bovine embryos using an improved air dry caryotyping method. Theriogenology. 2005 Jun;63(9):2434-2444.
10.White KL, Woods GL, Vanderwall DK, Li GP, Sessions BR, Bunch TD. 2004. Why clone horses and mules? Lessons learned from the successful births of cloned mules from a horse. IEEE Engineering in medicine and Biology Magazine 23 (2): 32-36.
11.Guang-Peng Li, Thomas D. Bunch, Kenneth L. White, Kenneth I. Aston, Lora N. Meerdo, Barry J. Pate, Benjamin R. Sessions. 2004. Development, chromosomal composition and allocation of inner cell mass and trophectoderm of bovine nuclear transfer blastocysts after culture in conditioned media. Mol Reprod Dev 68:189-197.
12.Guang-Peng Li, Kenneth L. White and Thomas D. Bunch. 2004. Review of enucleation methods and procedures used in animal cloning: state of the art. Cloning and Stem Cells 6:5-14.
13.G-P Li, White KL, Aston KI, Meerdo LN, Bunch TD. 2004. Conditioned medium increases the polyploid cell composition of bovine somatic cell nuclear transferred blastocysts. Reproduction 127: 221-228.
14. Dirk K. Vanderwall, Gordon L. Woods, Kenneth I. Aston, Thomas D. Bunch, Guanpeng Li, Lora N. Meerdo and Kenneth L. White. 2004. Cloned horse pregnancies produced using adult cumulus cells. Reproduction, Fertility and Development 16(7) 675–679.
15.Guang-Peng Li, G. E. Seidel, Jr. and E. L. Squires. 2004. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology 61(6): 1077-1084.
16.Woods GL, White KL, Vanderwall DK, Li GP, Aston KI, Bunch TD, Meerdo LN, Pate BJ. 2003. A mule cloned from fetal cells by nuclear transfer. Science 301(5636):1063. Epub 2003 May 29.
17.G -P Li, G. E. Seidel Jr., and E. L. Squires. 2003. Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa. Theriogenology, 59:1143-1155.
18.Guangpeng Li, Dayuan Chen, Lian Li, Zhiming Han, GE Seidel Jr. 2002. Rabbit cloning: improved fusion rates using cytochalasin B in the fusion buffer. Mol Reprod Dev 61(2):187-191.
19.Guang-Peng Li, Da-Yuan Chen, Li Lian, Qing-Yuan Sun, Min-Kang Wang. Ji-Song Li, Zhi-Ming Han. 2001. Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplasmic sperm injection (ICSI). Mol Reprod Dev 58:180-185.
20.Guang-Peng Li, Da-Yuan Chen, Li Lian, Qing-Yuan Sun, Min-Kang Wang. 2001. Mouse-rabbit germinal vesicle transfer reveals that factors regulating oocytes meiotic progression are not species-specific in mammals. J Exp Zool 289:322-329.
21.Guangpeng Li, Lian Li, Minkang Wang, Dayuan Chen. 2001. Maturation of the reconstituted oocytes by germinal vesicle transfer in rabbits and mice. Theriogenology 56:855-866.
22.Wang MK, Chen DY, Liu JL, Li GP, Sun QY. 2001. In vitro fertilization of mouse oocytes reconstructed by transfer of metaphase II chromosomes results in live births. Zygote 9:9-14.
23.Wang MK, Liu JL, Li GP, Lian L, Chen DY. 2001. Sucrose pretreatment for enucleation: An efficient and non-damage method for removing the spindle of the mouse MII oocyte. Mol Reprod Dev 58:432-436.
24.Guang-Peng Li, Jing-He Tan, Qing-Yuan Sun, Qing-Gang Meng, Kui-Zhong Yue, Xin-Sen Sun, Zi-Yi Li, Hong-Bin Wang, Li-Bin Xu. 2000. Cloned piglets born after nuclear transplantation of embryonic blastomeres into porcine oocytes matured in vivo. Cloning, 2(1): 45-52.
25.Chen DY, Sun QY, Liu JL, Li GP, Lian L, Wang MK, Han ZM, Song XF, Li JS, Sun Q, Chen YC, Zhang YP and Ding B. 1999. The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg. Science in China (Series C), 42:346-353.
26.Li Guang-Peng, Meng QG, Wei P, Yu YS, Chang ZL, Tan JH. 2001. Effects of cycloheximide (CHX) on in vitro maturation of porcine oocytes. Acta Zoologica Sinica 47(6): 684-690.
27.Li GP, Lian L, Lian Y, Chen DY. 2001. Reproduction engineering of domestic cat and nondomestic felid species. Zoological Research 22:413-420.
28.Li GP, Lan GC, Liu Y, Yu YS, Sun XS, Ma SF, Tan JH. 2000. Interspecific nuclear transfer between mouse and pig. Zoological Research 21:416-418.
29.Li GP. Lan GC, Ma SF, Tan JH. The behavior of the cell membrane of porcine oocytes matured in vitro in the electric field. Acta Anatomica Sinica, 2000, 21:145-148.
30.Li GP, Meng QG, Wei P, Tan JH. Technical modifications of in vitro maturation of porcine oocytes. Chinese Journal of Veterinary Science, 2000, 12: 32-35.
31.Li GP Cai SX, Xu LB, Tan JH. 2000. Studies on the early development of mouse tetraploid embryos produced by electrofusion. Developmental & Reproductive Biology, 7(1): 17-23.

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